Multi-modal Single-molecule Imaging with Continuously Controlled Spectral-resolution (CoCoS) Microscopy
Dear All,
You are cordially invited to the LMI seminar, that will be broadcasted on, Nov 4, 2020 01:00 PM Jerusalem
The speakers : Jonathan Jeffet, Group of Prof. Yuval Ebenstein - Department of Physical Chemistry Tel Aviv University
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https://us02web.zoom.us/j/85782221766?pwd=KzkySDF4Q1JGbjQwYVhUalBRQVh1QT09
Meeting ID: 857 8222 1766
Passcode: 142731
Multi-modal Single-molecule Imaging with Continuously Controlled Spectral-resolution (CoCoS) Microscopy
Jonathan Jeffet, Group of Prof. Yuval Ebenstein
Department of Physical Chemistry
Tel Aviv University
Abstract:
Color is a fundamental contrast mechanism in fluorescence microscopy, providing the basis for numerous imaging and spectroscopy techniques. The ever-growing need to acquire high-throughput, dynamic data from multicolor species is driving the development of optical schemes that optimize the achievable spectral, temporal, and spatial resolution needed in order to follow biological, chemical and physical processes. Here we introduce Continuously Controlled Spectral-resolution (CoCoS) microscopy, an imaging scheme that encodes color into spatial read-out in the image plane, with continuous control over the spectral resolution. The concept enables single-frame acquisition of multiple color channels, allowing simultaneous, single-molecule colocalization for barcoding and Förster resonance energy transfer (FRET) experiments. The simple control over the spectral dispersion allows switching between imaging modalities at a click of a button. We demonstrate the utility of CoCoS for multicolor localization microscopy of microRNA barcodes in clinical samples, single-molecule FRET measurements, and single-molecule spectroscopy. CoCoS may be integrated as a simple add-on to existing microscopes and will find use in applications that aim to record dynamic, multicolor localization events such as in multiplex FRET and tracking of multi-component, interacting complexes.
